LYSOZYME FROM CHICKEN EGG WHITE, TYPE VI MOLECULAR BIOLOGY GR,3X CRYSTALLIZED
lysozyme from human neutrophils
CAS: 9001-63-2;12650-88-3
Molecular Formula: C11H20NO6-
LYSOZYME FROM CHICKEN EGG WHITE, TYPE VI MOLECULAR BIOLOGY GR,3X CRYSTALLIZED - Names and Identifiers
LYSOZYME FROM CHICKEN EGG WHITE, TYPE VI MOLECULAR BIOLOGY GR,3X CRYSTALLIZED - Physico-chemical Properties
| Molecular Formula | C11H20NO6- |
| Molar Mass | 262.2796 |
| Appearance | powder |
| Color | white |
| Merck | 13,5660 |
| Storage Condition | -20°C |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| MDL | MFCD00131557 |
| Physical and Chemical Properties | Raw material processing from fresh egg white to get an enzyme product that can decompose mucopolysaccharides, white or slightly white crystalline or I set powder, odorless, sweet. Easily soluble in water, insoluble in acetone and ether. It is very stable in acidic solution, and the aqueous solution is easily destroyed by alkali. The optimum Ph is 6.6, and the isoelectric point pI = 10.5~11. The activity is not destroyed when the heat resistance is 550. Lysozyme is a basic protein. Often combined with chloride ions to form lysozyme chloride. |
| Use | Enzymes, can participate in the metabolism of mucopolysaccharides |
LYSOZYME FROM CHICKEN EGG WHITE, TYPE VI MOLECULAR BIOLOGY GR,3X CRYSTALLIZED - Risk and Safety
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S23 - Do not breathe vapour. S24/25 - Avoid contact with skin and eyes. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
| WGK Germany | 3 |
| RTECS | OL5989850 |
| FLUKA BRAND F CODES | 3-10 |
LYSOZYME FROM CHICKEN EGG WHITE, TYPE VI MOLECULAR BIOLOGY GR,3X CRYSTALLIZED - Nature
Open Data Verified Data
- An enzyme product that can decompose mucopolysaccharide obtained from fresh egg white of this strain is an alkaline protein, which is often combined with chloride ions to form lysozyme chloride. This product is white or white crystalline or amorphous powder, odorless, sweet. Soluble in water, insoluble in acetone, ether. In acidic solution is very stable, heat resistance to more than 55 deg C. The aqueous solution is easy to be destroyed by alkali. The optimum pH was 6.6 and the isoelectric point pl was 10. 5~11.
- egg white lysozyme consists of 129 amino acid residues arranged to form a single peptide chain with four pairs of disulfide bonds, with a molecular weight of 14388 and a flat long ellipsoid. It is a basic globulin with a high proportion of basic amino acids, acylamino residues and aromatic amino acids such as tryptophan. The active center of the enzyme is day 52 and Valley 35, which can hydrolyze the "p-i,4-" glycosidic bond between N-acetyl teichoic acid and N-acetyl glucosamine in mucopolysaccharide or chitin, it also functions as a transglutaminase. It can catalyze the hydrolysis of gram-positive bacteria cell wall insoluble polysaccharide to produce soluble mucopeptides, is a natural anti-infective substance with bactericidal effect, has antibacterial, antiviral, hemostatic, detumescence, analgesia and accelerate tissue repair and other functions.
- in nature, lysozyme is commonly found in the egg white of birds and poultry. Mammalian nasal discharge, saliva, tears, milk, urine, plasma, lymph, semen and nasal mucosa, intestinal tract, parotid gland, skin, white blood cells, lung, liver, spleen, in the cells of the kidney and the plants of cabbage, radish}, figs, papaya, barley, egg white is the most abundant, egg white contains about 0. 3%.
Last Update：2024-01-02 23:10:35
LYSOZYME FROM CHICKEN EGG WHITE, TYPE VI MOLECULAR BIOLOGY GR,3X CRYSTALLIZED - Preparation Method
Open Data Verified Data
- lysozyme has a positive charge in a wide pH range below the isoelectric point, and can be adsorbed on a weakly acidic cation exchange resin. After elution, the Lysozyme can be precipitated, after refining, a finished product can be obtained.
- 80kg of fresh egg white was added to 724 of the treated resin at 5N10 ° C., and the mixture was stirred and adsorbed for 6H. The mixture was allowed to stand at 0-5 ° C. Overnight. Pour out the upper layer of egg white, resin centrifugal spin drying, with distilled water to wash away the adhesion of egg protein, and then put the resin into the column, with 0. The resin was washed with about 150L of a phosphate buffer solution of 15mol/L and pH 10%, and eluted with about ammonium sulfate solution to collect the eluate. Ammonium sulfate was added to the eluate so that the final ammonium sulfate content was 40%, and a white precipitate was formed, which was left overnight in a cold place. Siphon the supernatant, precipitate suction filter suction dry, and then 1 times distilled water to dissolve the precipitate in a thin paste, and then into the dialysis bag, under the condition of about 5 degrees Celsius, the distilled water dialysis 24h or so, change water for 2-3 times. The precipitate was removed by centrifugation and washed once with a small amount of water. The washing liquid was combined with the centrifuged liquid. Then, 1mol/L sodium hydroxide solution was slowly added to the dialysis clear solution, while stirring continuously, so that the pH was raised to 8.0~9.0, and if there was white precipitate, it was removed by centrifugation. Then the pH was adjusted to 5.0 with 3mol/L hydrochloric acid, and freeze-dried to obtain white plate-like lysozyme. Can also be centrifuged with 3mol/L hydrochloric acid pH 3.5, slowly add 5% of solid sodium chloride under stirring, at a temperature of about 5 deg C for 48h, the precipitate was washed with acetone at 0 °c and dried to obtain lysozyme.
Last Update：2022-01-01 11:11:12
LYSOZYME FROM CHICKEN EGG WHITE, TYPE VI MOLECULAR BIOLOGY GR,3X CRYSTALLIZED - Use
Open Data Verified Data
- enzyme drugs. Involved in mucopolysaccharide metabolism. For sinusitis, acute pharyngitis, lichen planus, but also for chronic rhinitis, chronic pharyngitis and flat warts.
- usage and dosage of oral, once 30~50mg, one day 90~150mg. Clinical also for dental and urological surgery to stop bleeding and combined with antibiotics in the treatment of various bacterial and viral infections. Treatment of herpes zoster and flat warts, oral enteric-coated tablets, each time 50 ~ lOOmg, 3 times a day. It should be used abroad at 1-2g per day.
- preparation (1) buccal tablets;(2) enteric-coated tablets.
Last Update：2022-01-01 11:11:12
LYSOZYME FROM CHICKEN EGG WHITE, TYPE VI MOLECULAR BIOLOGY GR,3X CRYSTALLIZED - Safety
Open Data Verified Data
- Nausea, Vomit, Head Pain, itching, dizziness, Arthralgia, Diarrhea, loss of appetite and rash. Rare anaphylactic shock. Chicken protein allergy should not be used.
- light-shielding, closed, stored in a cool place.
Last Update：2022-01-01 11:11:13
LYSOZYME FROM CHICKEN EGG WHITE, TYPE VI MOLECULAR BIOLOGY GR,3X CRYSTALLIZED - Vitality
≥20000u/mg。
Last Update：2023-08-16 21:32:38
LYSOZYME FROM CHICKEN EGG WHITE, TYPE VI MOLECULAR BIOLOGY GR,3X CRYSTALLIZED - Reference Information
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| uses | Lysozyme can be involved in the metabolism of mucopolysaccharides, which can catalyze the hydrolysis of insoluble polysaccharides from Gram-positive bacteria cells to produce soluble mucopeptides, it is a kind of natural infectious substance with bactericidal effect, which has the functions of antibacterial, antiviral, hemostasis, detumescence, analgesia and accelerating tissue repair. Commonly used in acute pharyngitis, sinusitis, oral ulcers, lichen planus, but also for the treatment of chronic rhinitis, chronic pharyngitis and flat warts. However, the drug occasional Nausea, Vomit, Head Pain, itching, dizziness, Arthralgia, Diarrhea, loss of appetite and rash, etc., rare anaphylactic shock. Chicken protein allergy should not be used. Lysozyme Buccal Tablets: oral, 20mg, 4-6 times a day. Lysozyme Entric-Coated Tablets: herpes zoster and flat wart, oral, 50 ~ 100mg each time, 3 times a day. Topical 30~50mg, 3 times a day. It is mainly used for a variety of mucosal diseases in five departments, such as sinusitis, chronic rhinitis, pharyngitis, lichen planus, oral ulcer, exudative otitis media, etc, flat wart and chromium rhinitis. is an enzyme drug, can participate in the metabolism of mucopolysaccharides lysozyme is a kind of low molecular weight, Is an alkaline enzyme that can hydrolyze mucopolysaccharides in pathogenic bacteria. Lysozyme is a component of the immune defense mechanism of the normal body. It is named for its ability to dissolve the cell wall of bacteria. The cell wall insoluble mucopolysaccharides are decomposed into soluble glycopeptides mainly by disrupting the β-1,4 glycosidic bond between N-acetylmuramic acid and N-Acetylglucosamine in the cell wall, this results in the escape of the contents of the cell wall rupture and lysis of the bacteria. In the human body, lysozyme exists in neutrophils, monocytes and macrophages. It also exists in the mucous membrane secretion and becomes one of the body surface defense factors. |
| production method | was prepared by extraction from egg white. 1. Raw material processing fresh or frozen egg white (or eggshell water) 70kg, with test paper to measure its pH value, should be at about 8.0, after thawing through the lead sieve, remove the umbilical cord in the egg white, eggshell fragments and other impurities. 2. Adsorption with ice water cooling and stirring the egg white, the temperature is reduced to 5 deg C, slowly add 724 resin, stirring, the resin is completely suspended in the egg white. The temperature was maintained at 0-10 °c and stirring was continued for an additional 5H. The egg white was transferred into the cold storage, and the liquid was allowed to stand at 0-10 °c. Wash, elution next day, the upper layer of egg white clear liquid poured out, the lower layer with water to wash away the attached protein, successively washed four times. Finally, the resin was suction filtered to remove water, and 24kg of 0.15m phosphate buffer (pH 6.5) was added to the resin and stirred three times. After each stirring for 15min, the water was suction filtered off. The lysozyme was then eluted 4 times with 18kg of 10% ammonium sulfate. The eluates were combined, and 32% (weight/volume) solid ammonium sulfate was added to a content of 40% to precipitate a white precipitate. After standing overnight in the cold, the precipitate was filtered off. 4. Dialysis the precipitate was dissolved in 1.5L of distilled water and dialyzed for 24-36H in the cold storage to remove most of the ammonium sulfate. 5. Salting out dialysis is completed, and the solution is filtered with filter paper to obtain a clear dialysate. Slowly add sodium hydroxide solution with a dropper to raise the pH to 8.5-9.0. If there is a white precipitate, it should be removed by centrifugation. 3N hydrochloric acid was then added with stirring to bring the pH of the solution to 3.5. 5% solid sodium chloride was slowly added by volume to form a white precipitate. In 0-10 deg C cold storage for 48h, Suction filtration obtained lysozyme precipitation. 6. Add 10 times the amount of 0 ° C. Anhydrous acetone to the dry precipitate, stir to make the particles loose and fine, and let it stand in a cold place for several hours, and filter off the acetone. Precipitation with phosphorus pentoxide vacuum drying, namely oral or external lysozyme powder raw material. Without acetone dehydration, the lysozyme dialysate can be freeze-dried to obtain a sodium chloride-free lysozyme product. The yield was 0.1% by weight of egg white. raw material treatment take fresh or frozen egg white 70kg, so that the pH is about 8, thaw and sieve, remove the umbilical cord, eggshell fragments and other impurities in the egg white. Make the temperature drop to about 50 (not more than 100), add 724 resin (wet weight) while stirring, keep the resin in the egg white, keep it at 0~50, stir and absorb for 5H, then move the egg white into the cold storage 0 ~ 50, standing overnight. Egg white 724 resin; 30C → adsorption resin adsorbent to remove impurity protein, elution, precipitation, pour out the supernatant, wash with water to remove the protein attached to the surface of the resin, slowly wash 4 times, to prevent loss of tree fingers. The resin was suction-filtered to remove moisture. 24L, pH 6.5 phosphoric acid (0.15mol/L) buffer solution was added to the resin for 3 times, stirred for about 15min after each addition, and water was removed by Suction filtration under reduced pressure after stirring. Then 18L ammonium sulfate (10% g/L), 4 times of elution of lysozyme, each time after adding stirring 0.5h, filter dry. Combine the eluate, and add 320g of solid ammonium sulfate per liter of eluate to make the content 400g/L(40%). At this time, white precipitate was produced, which was left to stand overnight in cold place, and the supernatant was siphered the next day, the precipitate was centrifuged to obtain crude product. Resin adsorbate H2O; Phosphate buffer solution → eluent (NH4)^ 2 ^ SO4 → eluent (NH4)2SO4 → precipitate crude product dialysis the crude product was added to 1.5kg distilled water, completely dissolved, The dialysate was put into a dialysis bag and dialyzed in a cold storage for 24-36H to remove most of the ammonium sulfate to obtain a dialysate. Crude distilled water; Dissolve → dialysis dialysate salting out the dialysate to be clarified, slowly adding sodium hydroxide (1mol/L) Dropwise under constant stirring, neutralizing excess acid, and bringing the pH value to 8.5~9, if there is precipitation, it should be immediately removed by centrifugation, and then hydrochloric acid (3mol/L) is added under constant stirring, so that the pH of the solution reaches 3.5, according to the volume of each 100ml acidic dialysate slowly add solid sodium chloride 5G, that is, there is a white precipitation, in 0~50 Cold Storage placed 2d, centrifugal or filtered to lysozyme precipitation. Dialysate ^... → NaCl salting-out wet lysozyme is dried with continuous stirring, and 10 times the amount of anhydrous acetone cooled to 00C is added according to the mass of lysozyme precipitation, and the particle weight is fine, after standing in a cold place for several hours, the acetone was filtered off, and the precipitate was dried under vacuum until there was no acetone odor, and the lysozyme raw material for oral or external use was obtained. Wet lysozyme acetone; 00C → dry lysozyme preparation take dry crushed sugar powder, add 50g of talc powder per kg of the sugar powder (that is, 5%), pass through a 129 mesh sieve, add an appropriate amount of starch slurry (50g/L, 5%), stir evenly in the mixer to make soft material, pass through 12 mesh sieve, dry at 700C, and use 14 mesh sieve to make whole granules, moisture should be controlled at about 2% ~ 4%. Add a certain amount of lysozyme powder mixture, add 1% stearic acid enzyme, 16 mesh sieve 2 times, tablet, that is lysozyme buccal tablets, each containing lysozyme 20mg. Lysozyme tablets |
Last Update：2024-04-09 19:05:15
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CAS: 9001-63-2
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